JNT517-101

The goal of Parts A and B of this Phase 1, first-in-human, randomized study is to assess the safety, tolerability, and pharmacokinetics (PK) of single (SAD) and multiple (MAD) ascending doses of oral JNT-517 in healthy participants. In Part C, the goal is to evaluate the differences in bioavailability between a tablet and suspension formulation of JNT-517 and the food effect in healthy volunteers. All participants in Part C will receive JNT-517. The goal of Part D is to assess the safety, tolerability, PK, and effect on urinary Phe and other amino acids of JNT-517 in participants with phenylketonuria (PKU). Participants in Part D will receive either JNT-517 or placebo and will be blinded to their treatment assignment. The study consists of 4 parts: - Part A: SAD in healthy participants -randomized, double-blind, placebo-controlled - Part B: MAD in healthy participants (14 days)-randomized, double-blind, placebo-controlled - Part C: Relative bioavailability of 2 formulations and food effect in healthy participants-randomized, open-label - Part D: Phase 1b in participants with PKU (4 weeks)-randomized, double-blind, placebo-controlled In each part, participants will complete a Screening Period, a Treatment Period, and a Follow-up Period for safety.

Key Inclusion Criteria:

Parts A, B, and C:

1. Males and females 18 to 55 years of age.

2. Medically healthy with no clinically significant medical history.

3. Body mass index (BMI) of 18-40 kg/m2 and total body weight >50 kg (110 lbs).

4. Non-smoker for at least 2 weeks prior to dosing and willing to abstain during the
study.

Part D:

5. Males and females 18 to 65 years of age, inclusive.

6. Diagnosis of PKU with a confirmed genotype.

7. At least 2 plasma Phe levels >600 μM over the past 12 months.

8. BMI of 18-40 kg/m2.

All Parts:

9. Females of childbearing potential must agree to use 2 highly effective contraceptive
methods.

10. Capable of giving signed informed consent and able to comply with study procedures.

Key Exclusion Criteria:

All Parts:

1. Any acute or chronic medical condition that would prevent the participant from
complying with the procedures or place the participant at risk if they participate
in the study.

2. Positive for hepatitis B or C or human immunodeficiency virus.

3. Any history of malignancy in the last 5 years, excluding non-melanoma skin cancer.

4. Any history of liver disease.

5. Any surgical or medical conditions that may affect study drug absorption,
distribution, metabolism, or excretion.

6. Participation in another investigational drug trial within 30 days or, if known, 5
half-lives of the investigational drug (whichever is longer).

7. History of drug/alcohol abuse in the last year.

8. Current, recent, or suspected infection within 4 weeks of Screening of
SARS-CoV-2/COVID-19.

9. Received a vaccine for SARS-CoV-2/COVID-19 within 14 days of Screening.

10. Unable to tolerate oral medication.

11. Allergy to JNT-517 or any component of the investigational product.

12. Received >50 mL of blood or plasma within 30 days of Screening or >500 mL of blood
or plasma within 60 days of Screening.